Is important for all Affinity Screening experiment templates. All wells that are part of a serial dilution should have the same Dilutionseries ID.Although TRIC is very robust against such buffer mismatches, it could affect the measurements. Failure to do this, could bias the measured data with a gradient in salt, DMSO, glycerol or other additives. The buffer in tube/well 1 and subsequent tubes/wells must be the same. Always mix small volumes with a pipette.Serial dilutions are also commonly used to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution. Dilution is the process of making a solution weaker or less concentrated. Always use the smallest micro reaction tubes possible (e.g. Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes (for a standard curve for example). The high surface to volume ratio leads to surface adsorption even for well-behaved proteins. 500 μl or more) and then transfer into the Dianthus microwell plate. 20 μl) in large micro reaction tubes (e.g. Do not prepare less than 20 μl of sample.For ligands that are stored in DMSO, dilution is either performed in a way that maintains a constant level of DMSO throughout the dilution series or they are diluted in 100% DMSO and then each dilution point is diluted into buffer individually. The dilution factor can be freely determined by the user and is often adapted to obtain more data points for a transition phase or to cover a broader concentration range with fewer dilution points. This way, the ligand concentration is reduced by 50% in each dilution step. A number of spread plates is needed because the exact number of live bacteria in the sample is usually unknown. 10-2 to 10-8 ) is normally performed on the sample culture and spread plates created from the dilutions. Typically, a serial 1:1 dilution (dilution factor 2 in DI.Control software) is performed by transferring one volume of ligand solution to an equal volume of buffer, mixing, and repeating this step. Figure 1: Serial dilution series and plating.
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